PCR diagnosis of infections of the genitourinary system
Analyses And Studies / / April 21, 2016
- What is PCR?
- How to conduct research?
- What can be detected by PCR?
gonorrhea, trichomoniasis and acquire new features: instead of a bright clinical picture, with burning and a lot of unpleasant secretions, they hronizuyutsya immediately and imperceptibly flowing sluggishly.To some extent this is due to an uncontrolled intake of antibiotics.Microorganisms develop resistance to them, changing their properties drastically.As a result, passing STI antibiotics are not fully, the person becomes a source of unusual infections, which are extremely difficult to detect without analyzes.
Science does not stand still, and along with the change in the structure of pathogens takes place the development of improved diagnostic methods.PCR - polyme
What is PCR?Any microorganism
contains in its structure a DNA or RNA molecule - hereditary information.She needed it for further propagation, growth and development.The PCR method is able to detect the genetic information of any infectious diseases.It's enough to have at least one such molecule and test material that speaks about the unique sensitivity of the test.
Discovered pathogen DNA or RNA is synthesized by a specific enzyme repeatedly.As a result, the number of molecules of genetic information becomes visible to the recording system, and it gives a positive result.If the genetic material of a microorganism in the tissue there is, the result is negative.PCR diagnostics is valuable precisely: the risk of false-positive or false-negative responses is minimal.
Distinguish PCR diagnosis:
- quality - it can detect the DNA or RNA of the pathogen in the material, in principle, it is used for primary diagnosis.
- quantitative - is taken into account the number of copies of DNA or RNA in 1 ml of the mother.Accordingly, the more the microorganisms contained in it, the greater the number of copies as a result.The method is usually used to monitor the effectiveness of treatment.
How to conduct research?
for PCR diagnosis of inflammatory diseases of the urinary tract epithelium scraping use of the urethra, cervix, vagina, postoperative and biopsy material - small pieces of organs, urine.Before any analysis is recommended to abstain from sexual intercourse for at least 24 hours, men also can not urinate in 3 hours prior to the study.For women last rule applies only if the material is drawn out of the urethra.
Scraping is performed by a special thin brush, which is introduced into the urethra.Gynecologist cranks it several times to exfoliate cells from mucosal surfaces - most STD pathogens are parasites in them.In marked inflammation procedure may seem painful, but it lasts less than a minute.Production time analysis - a few hours, although the laboratory is usually issues a conclusion after 1-3 days, depending on its distance from the hospital and congestion.
What can be detected by PCR?
PCR diagnostics used to detect:
- genital herpes;
- human papillomavirus;
Often the doctor together with the search for the causative agent in the urogenital tract appoint a blood test for antibodies to it though.Joint interpretation of the data obtained allows him to judge the duration of the disease, its activity and assign the most appropriate therapy.An annual survey will help in time to reveal the hidden asymptomatic infections and prevent their negative consequences in the form of infertility and sexual dysfunction.